Journal: International Journal of Molecular Sciences

Article Title: Thyroid Hormone Upregulates Cav1.2 Channels in Cardiac Cells via the Downregulation of the Channels’ β4 Subunit

doi: 10.3390/ijms251910798

Figure Lengend Snippet: Relative mRNA expression and α1c protein abundance in H9c2 cells after incubation with T3. ( A , B ) Means ± SEMs of relative α1c subunit mRNA expression and protein abundance in H9c2 cells from three separate experiments. Insets show representative blots under control conditions (lanes a, c, e, and g) and 2, 6, 24, and 48 h after T3 treatment (lanes b, d, f, and h, respectively). Tubulin bands were used to normalize α1c subunit density values. In panels ( A , B ) each symbol represents a separate experiment. Original blots are presented in . * p < 0.05. ** p < 0.01.

Article Snippet: The antibody sources were polyclonal anti-rabbit α1c (ACC-003, 1:200; Alomone Labs, Jerusalem, Israel) and polyclonal anti-rabbit CACNB4 (1:500, 1:1000, A9304; ABclonal Technology, Woburn, MA, USA).

Techniques: Expressing, Incubation, Control

Journal: International Journal of Molecular Sciences

Article Title: Thyroid Hormone Upregulates Cav1.2 Channels in Cardiac Cells via the Downregulation of the Channels’ β4 Subunit

doi: 10.3390/ijms251910798

Figure Lengend Snippet: Relative α1c mRNA expression and protein abundance in H9c2 cells after Cavβ4 subunit overexpression. ( A ) Means ± SEMs of relative Cavβ4 protein abundance under control conditions and after Cavβ4 overexpression ( n = 3). The inset shows representative blots of the Cavβ4 subunit and GAPDH bands; the latter was used to normalize Cavβ4 subunit density values. Original blots are presented in . ( B ) Means ± SEMs of relative α1c subunit mRNA expression from control and Cavβ4 overexpression experiments ( n = 14). ( C ) Means ± SEMs of α1c protein abundance from control and Cavβ4 overexpression experiments ( n = 10). The inset shows representative blots of α1c subunit and tubulin bands under control conditions (lanes a and c) and after Cavβ4 overexpression (lanes b and d) from two separate experiments. Original blots are presented in . In panels ( A , B , C ), each symbol represents a separate experiment. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The antibody sources were polyclonal anti-rabbit α1c (ACC-003, 1:200; Alomone Labs, Jerusalem, Israel) and polyclonal anti-rabbit CACNB4 (1:500, 1:1000, A9304; ABclonal Technology, Woburn, MA, USA).

Techniques: Expressing, Over Expression, Control

Journal: International Journal of Molecular Sciences

Article Title: Thyroid Hormone Upregulates Cav1.2 Channels in Cardiac Cells via the Downregulation of the Channels’ β4 Subunit

doi: 10.3390/ijms251910798

Figure Lengend Snippet: Role of Cavβ4 in pCREB regulation by T3. Schematic showing signaling mechanisms hypothetically involved in thyroid hormone actions on the CACNA1C gene via pCREB and the release of Cavβ4 inhibition of α1c subunit expression in ventricular cardiac cells.

Article Snippet: The antibody sources were polyclonal anti-rabbit α1c (ACC-003, 1:200; Alomone Labs, Jerusalem, Israel) and polyclonal anti-rabbit CACNB4 (1:500, 1:1000, A9304; ABclonal Technology, Woburn, MA, USA).

Techniques: Inhibition, Expressing